rabbit phospho smad Search Results


smad5  (Cell Signaling Technology Inc)
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Smad5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti p smad2  (Cell Signaling Technology Inc)
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Anti P Smad2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho smad1  (Cell Signaling Technology Inc)
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Anti Phospho Smad1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti p smad3  (Cell Signaling Technology Inc)
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Anti P Smad3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated mothers against decapentaplegic homolog 2 smad family member 2  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc phosphorylated mothers against decapentaplegic homolog 2 smad family member 2
Phosphorylated Mothers Against Decapentaplegic Homolog 2 Smad Family Member 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho smad3 s423 425  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc phospho smad3 s423 425
Figure 2. cDCs and cDC progenitors: CD115+ CDP (Lin2CD117int), SiglecH2Ly6C+, and SiglecH2Ly6C2 pre-DCs are increased in Smad32/2 mice. Immunophenotyping of <t>Smad3−/−(black</t> circles) or Smad3+/+ (white circles) mice (n = 6/genotype) was performed using flow cytometry. (A) Representative contour plots show CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, spleens (SP), superficial lymph nodes, and mesenteric lymph nodes. Dots in the graphs show the numbers of CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, SP, superficial lymph nodes, and mesenteric lymph nodes of each mouse. Horizontal bars show means. (B) Representative contour plots show the expression of CD115/CD135 in Lin−Sca-1−CD117hi or Lin−Sca-1−CD117int gates. Dots in the graphs show the numbers of Lin−Sca-1−CD117hiCD115+CD135+ MDPs and Lin−Sca-1−CD117intCD115+CD135+ CDPs in the BM of each mouse. (C) Bar graphs show the expression levels of Cx3cr1 and Dngr1 mRNA in Lin−CD115+ BM cells detected using RT–qPCR with means + s.d. Black bars represent Smad3−/−, whereas white bars represent Smad3+/+ mice. Representative contour plots show CX3CR1+CD370+Lin−CD117intCD115+CD135+ BM cells. (D) Representative contour plots show the expression of SiglecH/Ly6C in the CD11c+MHCII−CD135+CD172α−pre-DC gate. Dots in the graphs show the numbers of CD11c+MHCII−CD135+CD172α−SiglecH−cells in BM. P-values were calculated by a two-tailed unpaired t test.
Phospho Smad3 S423 425, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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41d10 cell signaling technology  (Cell Signaling Technology Inc)
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Figure 2. cDCs and cDC progenitors: CD115+ CDP (Lin2CD117int), SiglecH2Ly6C+, and SiglecH2Ly6C2 pre-DCs are increased in Smad32/2 mice. Immunophenotyping of <t>Smad3−/−(black</t> circles) or Smad3+/+ (white circles) mice (n = 6/genotype) was performed using flow cytometry. (A) Representative contour plots show CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, spleens (SP), superficial lymph nodes, and mesenteric lymph nodes. Dots in the graphs show the numbers of CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, SP, superficial lymph nodes, and mesenteric lymph nodes of each mouse. Horizontal bars show means. (B) Representative contour plots show the expression of CD115/CD135 in Lin−Sca-1−CD117hi or Lin−Sca-1−CD117int gates. Dots in the graphs show the numbers of Lin−Sca-1−CD117hiCD115+CD135+ MDPs and Lin−Sca-1−CD117intCD115+CD135+ CDPs in the BM of each mouse. (C) Bar graphs show the expression levels of Cx3cr1 and Dngr1 mRNA in Lin−CD115+ BM cells detected using RT–qPCR with means + s.d. Black bars represent Smad3−/−, whereas white bars represent Smad3+/+ mice. Representative contour plots show CX3CR1+CD370+Lin−CD117intCD115+CD135+ BM cells. (D) Representative contour plots show the expression of SiglecH/Ly6C in the CD11c+MHCII−CD135+CD172α−pre-DC gate. Dots in the graphs show the numbers of CD11c+MHCII−CD135+CD172α−SiglecH−cells in BM. P-values were calculated by a two-tailed unpaired t test.
41d10 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phosphorylated smad 2 3  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti phosphorylated smad 2 3
Figure 2. cDCs and cDC progenitors: CD115+ CDP (Lin2CD117int), SiglecH2Ly6C+, and SiglecH2Ly6C2 pre-DCs are increased in Smad32/2 mice. Immunophenotyping of <t>Smad3−/−(black</t> circles) or Smad3+/+ (white circles) mice (n = 6/genotype) was performed using flow cytometry. (A) Representative contour plots show CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, spleens (SP), superficial lymph nodes, and mesenteric lymph nodes. Dots in the graphs show the numbers of CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, SP, superficial lymph nodes, and mesenteric lymph nodes of each mouse. Horizontal bars show means. (B) Representative contour plots show the expression of CD115/CD135 in Lin−Sca-1−CD117hi or Lin−Sca-1−CD117int gates. Dots in the graphs show the numbers of Lin−Sca-1−CD117hiCD115+CD135+ MDPs and Lin−Sca-1−CD117intCD115+CD135+ CDPs in the BM of each mouse. (C) Bar graphs show the expression levels of Cx3cr1 and Dngr1 mRNA in Lin−CD115+ BM cells detected using RT–qPCR with means + s.d. Black bars represent Smad3−/−, whereas white bars represent Smad3+/+ mice. Representative contour plots show CX3CR1+CD370+Lin−CD117intCD115+CD135+ BM cells. (D) Representative contour plots show the expression of SiglecH/Ly6C in the CD11c+MHCII−CD135+CD172α−pre-DC gate. Dots in the graphs show the numbers of CD11c+MHCII−CD135+CD172α−SiglecH−cells in BM. P-values were calculated by a two-tailed unpaired t test.
Anti Phosphorylated Smad 2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti p smad1 5 8 antibody  (Cell Signaling Technology Inc)
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Figure 2. cDCs and cDC progenitors: CD115+ CDP (Lin2CD117int), SiglecH2Ly6C+, and SiglecH2Ly6C2 pre-DCs are increased in Smad32/2 mice. Immunophenotyping of <t>Smad3−/−(black</t> circles) or Smad3+/+ (white circles) mice (n = 6/genotype) was performed using flow cytometry. (A) Representative contour plots show CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, spleens (SP), superficial lymph nodes, and mesenteric lymph nodes. Dots in the graphs show the numbers of CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, SP, superficial lymph nodes, and mesenteric lymph nodes of each mouse. Horizontal bars show means. (B) Representative contour plots show the expression of CD115/CD135 in Lin−Sca-1−CD117hi or Lin−Sca-1−CD117int gates. Dots in the graphs show the numbers of Lin−Sca-1−CD117hiCD115+CD135+ MDPs and Lin−Sca-1−CD117intCD115+CD135+ CDPs in the BM of each mouse. (C) Bar graphs show the expression levels of Cx3cr1 and Dngr1 mRNA in Lin−CD115+ BM cells detected using RT–qPCR with means + s.d. Black bars represent Smad3−/−, whereas white bars represent Smad3+/+ mice. Representative contour plots show CX3CR1+CD370+Lin−CD117intCD115+CD135+ BM cells. (D) Representative contour plots show the expression of SiglecH/Ly6C in the CD11c+MHCII−CD135+CD172α−pre-DC gate. Dots in the graphs show the numbers of CD11c+MHCII−CD135+CD172α−SiglecH−cells in BM. P-values were calculated by a two-tailed unpaired t test.
Rabbit Polyclonal Anti P Smad1 5 8 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho smad3  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc phospho smad3
Figure 2. cDCs and cDC progenitors: CD115+ CDP (Lin2CD117int), SiglecH2Ly6C+, and SiglecH2Ly6C2 pre-DCs are increased in Smad32/2 mice. Immunophenotyping of <t>Smad3−/−(black</t> circles) or Smad3+/+ (white circles) mice (n = 6/genotype) was performed using flow cytometry. (A) Representative contour plots show CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, spleens (SP), superficial lymph nodes, and mesenteric lymph nodes. Dots in the graphs show the numbers of CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, SP, superficial lymph nodes, and mesenteric lymph nodes of each mouse. Horizontal bars show means. (B) Representative contour plots show the expression of CD115/CD135 in Lin−Sca-1−CD117hi or Lin−Sca-1−CD117int gates. Dots in the graphs show the numbers of Lin−Sca-1−CD117hiCD115+CD135+ MDPs and Lin−Sca-1−CD117intCD115+CD135+ CDPs in the BM of each mouse. (C) Bar graphs show the expression levels of Cx3cr1 and Dngr1 mRNA in Lin−CD115+ BM cells detected using RT–qPCR with means + s.d. Black bars represent Smad3−/−, whereas white bars represent Smad3+/+ mice. Representative contour plots show CX3CR1+CD370+Lin−CD117intCD115+CD135+ BM cells. (D) Representative contour plots show the expression of SiglecH/Ly6C in the CD11c+MHCII−CD135+CD172α−pre-DC gate. Dots in the graphs show the numbers of CD11c+MHCII−CD135+CD172α−SiglecH−cells in BM. P-values were calculated by a two-tailed unpaired t test.
Phospho Smad3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphor smad2 3  (Cell Signaling Technology Inc)
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Figure 2. cDCs and cDC progenitors: CD115+ CDP (Lin2CD117int), SiglecH2Ly6C+, and SiglecH2Ly6C2 pre-DCs are increased in Smad32/2 mice. Immunophenotyping of <t>Smad3−/−(black</t> circles) or Smad3+/+ (white circles) mice (n = 6/genotype) was performed using flow cytometry. (A) Representative contour plots show CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, spleens (SP), superficial lymph nodes, and mesenteric lymph nodes. Dots in the graphs show the numbers of CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, SP, superficial lymph nodes, and mesenteric lymph nodes of each mouse. Horizontal bars show means. (B) Representative contour plots show the expression of CD115/CD135 in Lin−Sca-1−CD117hi or Lin−Sca-1−CD117int gates. Dots in the graphs show the numbers of Lin−Sca-1−CD117hiCD115+CD135+ MDPs and Lin−Sca-1−CD117intCD115+CD135+ CDPs in the BM of each mouse. (C) Bar graphs show the expression levels of Cx3cr1 and Dngr1 mRNA in Lin−CD115+ BM cells detected using RT–qPCR with means + s.d. Black bars represent Smad3−/−, whereas white bars represent Smad3+/+ mice. Representative contour plots show CX3CR1+CD370+Lin−CD117intCD115+CD135+ BM cells. (D) Representative contour plots show the expression of SiglecH/Ly6C in the CD11c+MHCII−CD135+CD172α−pre-DC gate. Dots in the graphs show the numbers of CD11c+MHCII−CD135+CD172α−SiglecH−cells in BM. P-values were calculated by a two-tailed unpaired t test.
Phosphor Smad2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho smad2  (Cell Signaling Technology Inc)
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Figure 2. cDCs and cDC progenitors: CD115+ CDP (Lin2CD117int), SiglecH2Ly6C+, and SiglecH2Ly6C2 pre-DCs are increased in Smad32/2 mice. Immunophenotyping of <t>Smad3−/−(black</t> circles) or Smad3+/+ (white circles) mice (n = 6/genotype) was performed using flow cytometry. (A) Representative contour plots show CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, spleens (SP), superficial lymph nodes, and mesenteric lymph nodes. Dots in the graphs show the numbers of CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, SP, superficial lymph nodes, and mesenteric lymph nodes of each mouse. Horizontal bars show means. (B) Representative contour plots show the expression of CD115/CD135 in Lin−Sca-1−CD117hi or Lin−Sca-1−CD117int gates. Dots in the graphs show the numbers of Lin−Sca-1−CD117hiCD115+CD135+ MDPs and Lin−Sca-1−CD117intCD115+CD135+ CDPs in the BM of each mouse. (C) Bar graphs show the expression levels of Cx3cr1 and Dngr1 mRNA in Lin−CD115+ BM cells detected using RT–qPCR with means + s.d. Black bars represent Smad3−/−, whereas white bars represent Smad3+/+ mice. Representative contour plots show CX3CR1+CD370+Lin−CD117intCD115+CD135+ BM cells. (D) Representative contour plots show the expression of SiglecH/Ly6C in the CD11c+MHCII−CD135+CD172α−pre-DC gate. Dots in the graphs show the numbers of CD11c+MHCII−CD135+CD172α−SiglecH−cells in BM. P-values were calculated by a two-tailed unpaired t test.
Phospho Smad2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. cDCs and cDC progenitors: CD115+ CDP (Lin2CD117int), SiglecH2Ly6C+, and SiglecH2Ly6C2 pre-DCs are increased in Smad32/2 mice. Immunophenotyping of Smad3−/−(black circles) or Smad3+/+ (white circles) mice (n = 6/genotype) was performed using flow cytometry. (A) Representative contour plots show CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, spleens (SP), superficial lymph nodes, and mesenteric lymph nodes. Dots in the graphs show the numbers of CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, SP, superficial lymph nodes, and mesenteric lymph nodes of each mouse. Horizontal bars show means. (B) Representative contour plots show the expression of CD115/CD135 in Lin−Sca-1−CD117hi or Lin−Sca-1−CD117int gates. Dots in the graphs show the numbers of Lin−Sca-1−CD117hiCD115+CD135+ MDPs and Lin−Sca-1−CD117intCD115+CD135+ CDPs in the BM of each mouse. (C) Bar graphs show the expression levels of Cx3cr1 and Dngr1 mRNA in Lin−CD115+ BM cells detected using RT–qPCR with means + s.d. Black bars represent Smad3−/−, whereas white bars represent Smad3+/+ mice. Representative contour plots show CX3CR1+CD370+Lin−CD117intCD115+CD135+ BM cells. (D) Representative contour plots show the expression of SiglecH/Ly6C in the CD11c+MHCII−CD135+CD172α−pre-DC gate. Dots in the graphs show the numbers of CD11c+MHCII−CD135+CD172α−SiglecH−cells in BM. P-values were calculated by a two-tailed unpaired t test.

Journal: Life science alliance

Article Title: Repression of SMAD3 by STAT3 and c-Ski induces conventional dendritic cell differentiation.

doi: 10.26508/lsa.201900581

Figure Lengend Snippet: Figure 2. cDCs and cDC progenitors: CD115+ CDP (Lin2CD117int), SiglecH2Ly6C+, and SiglecH2Ly6C2 pre-DCs are increased in Smad32/2 mice. Immunophenotyping of Smad3−/−(black circles) or Smad3+/+ (white circles) mice (n = 6/genotype) was performed using flow cytometry. (A) Representative contour plots show CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, spleens (SP), superficial lymph nodes, and mesenteric lymph nodes. Dots in the graphs show the numbers of CD11c+MHCII+, CD11b+CD11c+MHCII+, and CD8+CD11c+MHCII+ cells in BM, SP, superficial lymph nodes, and mesenteric lymph nodes of each mouse. Horizontal bars show means. (B) Representative contour plots show the expression of CD115/CD135 in Lin−Sca-1−CD117hi or Lin−Sca-1−CD117int gates. Dots in the graphs show the numbers of Lin−Sca-1−CD117hiCD115+CD135+ MDPs and Lin−Sca-1−CD117intCD115+CD135+ CDPs in the BM of each mouse. (C) Bar graphs show the expression levels of Cx3cr1 and Dngr1 mRNA in Lin−CD115+ BM cells detected using RT–qPCR with means + s.d. Black bars represent Smad3−/−, whereas white bars represent Smad3+/+ mice. Representative contour plots show CX3CR1+CD370+Lin−CD117intCD115+CD135+ BM cells. (D) Representative contour plots show the expression of SiglecH/Ly6C in the CD11c+MHCII−CD135+CD172α−pre-DC gate. Dots in the graphs show the numbers of CD11c+MHCII−CD135+CD172α−SiglecH−cells in BM. P-values were calculated by a two-tailed unpaired t test.

Article Snippet: For the PLA, fixed cells were permeabilized by 0.1% Triton X-100 in PBS, and stained with rabbit antibodies against SMAD2, SMAD3, phospho-SMAD2 (S465/467), phospho-SMAD3 (S423/425) (Cell Signaling Technology), STAT3 (clone C-20; Santa Cruz Biotechnology), c-SKI (clone 6D763; Santa Cruz Biotechnology), and FLAG (clone 8H8L17; Invitrogen).

Techniques: Cytometry, Expressing, Quantitative RT-PCR, Two Tailed Test

Figure 7. Interaction of c-SKI with R-SMADs and phosphorylated STAT3 is essential for repression of SMAD3 and cDC differentiation. (A) Effects of c-SKI mutations (Δ2/3 that does not interact with Smad2/3 and W274E that does not interact with Smad4) on the Smad3 promoter activity in CD11b+ FLT3L- induced or CD11b+ GM-CSF plus IL-4–induced BMDCs transfected with the indicated plasmids were determined by the Smad3 promoter luciferase reporter assay. (B) Effects of STAT3 phosphorylation site-specific mutants (Y705F and S727A) on SMAD2/3-induced activation of the Smad3 promoter constructs transfected with the indicated plasmids in CD11b+ FLT3L-induced or CD11b+ GM-CSF plus IL-4–induced BMDCs were determined by the Smad3 promoter luciferase reporter assay. (C) Contour plots show the expression of CD11c/MHCII and CD11b/CD24 in the CD11c+MHCII+ gate of FLT3L-induced or GM-CSF plus IL-4–induced BMDCs transfected with the indicated c-SKI mutants, c-SKI siRNA and control pcDNA or control siRNA. (D) Contour plots show the expression of CD11c/MHCII and CD11b/CD24 in the CD11c+MHCII+ gate of FLT3L- induced or GM-CSF plus IL-4–induced BMDCs transfected with the indicated STAT3 phosphorylation site-specific mutants (Y705F and S727A) and STAT3 siRNA or control siRNA 4 h before culture and analysed on day 8. Luciferase reporter assays were performed in triplicate. Data are representative of three independent experiments. Graphs show means + s.d. P-values were calculated by a two-tailed unpaired t test. ***P < 0.0005.

Journal: Life science alliance

Article Title: Repression of SMAD3 by STAT3 and c-Ski induces conventional dendritic cell differentiation.

doi: 10.26508/lsa.201900581

Figure Lengend Snippet: Figure 7. Interaction of c-SKI with R-SMADs and phosphorylated STAT3 is essential for repression of SMAD3 and cDC differentiation. (A) Effects of c-SKI mutations (Δ2/3 that does not interact with Smad2/3 and W274E that does not interact with Smad4) on the Smad3 promoter activity in CD11b+ FLT3L- induced or CD11b+ GM-CSF plus IL-4–induced BMDCs transfected with the indicated plasmids were determined by the Smad3 promoter luciferase reporter assay. (B) Effects of STAT3 phosphorylation site-specific mutants (Y705F and S727A) on SMAD2/3-induced activation of the Smad3 promoter constructs transfected with the indicated plasmids in CD11b+ FLT3L-induced or CD11b+ GM-CSF plus IL-4–induced BMDCs were determined by the Smad3 promoter luciferase reporter assay. (C) Contour plots show the expression of CD11c/MHCII and CD11b/CD24 in the CD11c+MHCII+ gate of FLT3L-induced or GM-CSF plus IL-4–induced BMDCs transfected with the indicated c-SKI mutants, c-SKI siRNA and control pcDNA or control siRNA. (D) Contour plots show the expression of CD11c/MHCII and CD11b/CD24 in the CD11c+MHCII+ gate of FLT3L- induced or GM-CSF plus IL-4–induced BMDCs transfected with the indicated STAT3 phosphorylation site-specific mutants (Y705F and S727A) and STAT3 siRNA or control siRNA 4 h before culture and analysed on day 8. Luciferase reporter assays were performed in triplicate. Data are representative of three independent experiments. Graphs show means + s.d. P-values were calculated by a two-tailed unpaired t test. ***P < 0.0005.

Article Snippet: For the PLA, fixed cells were permeabilized by 0.1% Triton X-100 in PBS, and stained with rabbit antibodies against SMAD2, SMAD3, phospho-SMAD2 (S465/467), phospho-SMAD3 (S423/425) (Cell Signaling Technology), STAT3 (clone C-20; Santa Cruz Biotechnology), c-SKI (clone 6D763; Santa Cruz Biotechnology), and FLAG (clone 8H8L17; Invitrogen).

Techniques: Activity Assay, Transfection, Luciferase, Reporter Assay, Phospho-proteomics, Activation Assay, Construct, Expressing, Control, Two Tailed Test